Journal: MedComm

Article Title: Mas‐Related G Protein‐Coupled Receptor Member D Sustains Hypertension

doi: 10.1002/mco2.70706

Figure Lengend Snippet: Effect of MrgD on blood pressure. (A) Flow chart of Ad‐MrgD‐treated SD rats; (B–D) the SBP, DBP, and MAP of Ad‐MrgD‐treated SD rats, n = 8 biological replicates per group. (E) Flow chart of MrgD shRNA‐treated SHR rats; (F–H) the SBP, DBP, and MAP of MrgD shRNA‐treated SHR rats, n = 8 biological replicates per group. (I) Flow chart of Ang II‐treated MrgD KO mice; (J–L) the SBP, DBP, and MAP of Ang II‐treated MrgD KO mice, n = 8 biological replicates per group. (M) Representative images of endothelial‐dependent (PFG2α and Ach) vasodilation and vasoconstriction responses. (N) Contraction effect curve of PGF2α‐induced mesenteric arteries, n = 6 biological replicates per group; (O) diastolic effect curve of Ach‐induced mesenteric arteries, n = 6 biological replicates per group. (P) Representative images of non‐endothelial‐dependent (NE and SNP) vasodilation and vasoconstriction responses. (Q) Contraction effect curve of NE‐induced mesenteric arteries, n = 6 biological replicates per group; (R) diastolic effect curve of SNP‐induced mesenteric arteries, n = 6 biological replicates per group. The data were expressed as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: Subsequently, vasoconstrictive responses were elicited by cumulative concentrations of PGF2α (Sigma, CAS 4510‐16‐1) or NE (MCE, HY‐13715) over a concentration range of 10 −9 to 10 −4 M. Endothelium‐dependent and ‐independent vasorelaxation were assessed with Ach (MCE, HY‐B0282) and SNP (MCE, HY‐B0564), respectively, using the same concentration range (10 −9 to 10 −4 M).

Techniques: shRNA

Journal: MedComm

Article Title: Mas‐Related G Protein‐Coupled Receptor Member D Sustains Hypertension

doi: 10.1002/mco2.70706

Figure Lengend Snippet: Risperidone alleviated hypertension and remodeling of the mesenteric artery. (A) The structure of risperidone; (B) the structure of risperidone binding with MrgD; (C–E) risperidone relieved Ang II‐induced hypertension in mice, * vs. Saline and # vs. Ang II, n = 8 biological replicates per group. (F and G) Risperidone reversed Ang II‐induced increases in the mRNA levels of collagen I and TGF‐β in the mesenteric artery of mice, n = 5 biological replicates per group. (H–K) Risperidone reversed Ang II‐induced changes in the protein levels of collagen I, TGF‐β, and SM‐22α in the mesenteric artery of mice, n = 5 biological replicates per group. (L) Representative images of endothelial‐dependent (PFG2α and Ach) and non‐endothelial‐dependent (NE and SNP) vasodilation and vasoconstriction responses; (M and N) risperidone reduced vasorelaxation in the mesenteric artery of mice treated with Ang II, * vs. Saline and # vs. Ang II, n = 6 biological replicates per group. (O and P) Risperidone reduced contraction in the mesenteric artery of mice treated with Ang II, * vs. Saline and # vs. Ang II, n = 6 biological replicates per group. The data were expressed as mean ± SEM. */# p < 0.05, **/## p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: Subsequently, vasoconstrictive responses were elicited by cumulative concentrations of PGF2α (Sigma, CAS 4510‐16‐1) or NE (MCE, HY‐13715) over a concentration range of 10 −9 to 10 −4 M. Endothelium‐dependent and ‐independent vasorelaxation were assessed with Ach (MCE, HY‐B0282) and SNP (MCE, HY‐B0564), respectively, using the same concentration range (10 −9 to 10 −4 M).

Techniques: Binding Assay, Saline

Journal: Frontiers in Physiology

Article Title: Ion channels and atrial fibrillation: mitophagy as a key mediator

doi: 10.3389/fphys.2025.1687578

Figure Lengend Snippet: In vivo experimental validation. (A–F) The (A) electrocardiogram, (B) hematoxylin-eosin, (C) Masson, (D) quantification of collagen volume fraction, (E) Sirius staining, and (F) quantification of Sirius red staining area percentage in rats with acetylcholine-calcium chloride-induced atrial tachyarrhythmias ( n = 5). (G) Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) to detect the relative expression levels of Hub genes, mitophagy, and ion channel-related genes ( n = 5). (H) Western blotting to detect the expression of BAX, TLR4, MIF, and GLUL proteins ( n = 3). Results are expressed as the mean ± S.D. * P < 0.05 vs. the control group, ** P < 0.01 vs. the control group. The normality of data distribution was assessed using the Shapiro-Wilk test, and the homogeneity of variances was verified by the Brown-Forsythe test. For comparisons among multiple groups that satisfied both normality and homogeneity of variances, a one-way analysis of variance (ANOVA) was employed, followed by Tukey’s honestly significant difference (HSD) post hoc test for specific group comparisons. For data that did not meet the assumptions of parametric tests, the non-parametric Kruskal-Wallis H test was used.

Article Snippet: Reagents: Acetylcholine Chloride (Ach) (Shanghai yuanye, Cat. S30170-5g); Anhydrous Calcium Chloride (CaCl 2 ) (Shanghai yuanye, Cat. S24110-500 g); Isoflurane (SAMVET, Lot.235180101); Hematoxylin-Eosin Constant Dye Kit (Servicebio, Cat. G1076); Masson Dye Solution Set (Servicebio, Cat. G1006); Differentiation Fluid (Servicebio, Cat. G1039); Sirius Red Staining Solution (Servicebio, Cat. G1018); Environmentally Friendly Dewaxing Liquid (Servicebio, Cat. G1128); Paraformaldehyde Fixative (Neutral) (Servicebio, Cat. G1101); 75% ethanol (Shenyang GuangCai, Cat.20250102); Absolute ethanol (Sinopharm Chemical Reagent Co.,Ltd., Cat.100092683); Xylene (Sinopharm Chemical Reagent Co.,Ltd., Cat.10023418); Normal Butanol (Sinopharm Chemical Reagent Co.,Ltd., Cat.100052190); Neutral Gum (Sinopharm Chemical Reagent Co.,Ltd., Cat.10004160); RNA Extraction Solution (Servicebio, Cat. G3013); chloroform Substitute (Servicebio, Cat. G3014); Isopropanol (Sinopharm Chemical Reagent Co.,Ltd., Cat.80109218); SweScript All-in-One RT SuperMix for qPCR (Servicebio, Cat. G3337); 2×Universal Blue SYnthetic Binding Reagent Green qPCR Master Mix (Servicebio, Cat. G3326); Bicinchoninic Acid Protein Concentration Measurement Kit (Beyotime, P0010); Radioimmunoprecipitation Assay Lysate (Beyotime, P0013B); Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) Gel Rapid Preparation Kits (Beyotime, P0015L); Maker (Epizyme Biotech, Cat. WJ103); β-Actin Rabbit mAb (Abclonal, Cat. AC038); Anti-MIF (UpingBio, Cat. YP-Ab-15949); Anti-GLUL (UpingBio, Cat. YP-Ab-12846); Anti-TLR4 (UpingBio, Cat. YP-Ab-13172); Anti-BAX (BOSTER, Cat. A00183); Goat Anti-Rabbit IgG (H + L), HRP (UpingBio, Cat. YP848537-H).

Techniques: In Vivo, Biomarker Discovery, Staining, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Expressing, Western Blot, Control